Enhanced Validation

Monoclonal Anti-SIX1 Antibody

SIX homeobox 1
References (2)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human SIX1
Alternative Gene Names
DFNA23
Open Datasheet
Price
$505.00
Product Number
AMAb90544
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[6 pictures]
Immunohistochemistry analysis in human skeletal muscle and liver tissues using AMAb90544 antibody. Corresponding SIX1 RNA-seq data are presented for the same tissues.
Show all images
[1 pictures]
Western blot analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SIX1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Show all images
[1 pictures]
Immunofluorescence staining in RH30 cell line with Anti-SIX1 monoclonal antibody, showing spotty nuclear staining in green. Microtubule probes are visualized in red (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
SIX homeobox 1
Target Gene
SIX1
Belongs to Antibody Panel
Epithelial to Mesenchymal (EMT) Markers
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000051367 (99%)

Rat ENSRNOG00000022777 (99%)

Clonality
Monoclonal
Clone ID
CL0185
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90544 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90544 on the Human Protein Atlas
Protein Name
SIX homeobox 1
Gene Name
SIX1
Alternative Gene Names
DFNA23
UniProt ID
Q15475
Gene (Ensembl)
ENSG00000126778
Entrez Gene ID
Entrez gene: 6495
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Saeki T, Yoshimatsu S, Ishikawa M, Hon CC, Koya I, Shibata S, Hosoya M, Saegusa C, Ogawa K, Shin JW, Fujioka M, Okano H

Critical roles of FGF, RA, and WNT signalling in the development of the human otic placode and subsequent lineages in a dish

Regen Ther , 2022 May 16; 20:165-186. Epub 2022 May 16

PubMed ID: 35620640 DOI: 10.1016/j.reth.2022.04.008

Washausen S, Knabe W

Lateral line placodes of aquatic vertebrates are evolutionarily conserved in mammals

Biol Open , 2018 May 30; 7(6):bio031815. Epub 2018 May 30

PubMed ID: 29848488 DOI: 10.1242/bio.031815

Did we miss your publication?

Have you published using AMAb90544? Please let us know and we will be happy to include your reference on this page.

Read more

Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

Read more

Alternative Antibodies
Anti-SIX1 (HPA001893)
Corresponding Antigens
PrEST Antigen SIX1 (APrEST70399)

Questions?

Our customer support and expert scientific support teams are here to help you succeed in your research.

Get in touch