Human Protein Atlas
Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000010188 (99%)
Mouse ENSMUSG00000038331 (99%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 1 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
Evaluating real-time immunohistochemistry on multiple tissue samples, multiple targets and multiple antibody labeling methods
BMC Res Notes , 2013 Dec 18; 6:542. Epub 2013 Dec 18
Scalable in situ hybridization on tissue arrays for validation of novel cancer and tissue-specific biomarkers.
PLoS One , 2012; 7(3):e32927. Epub 2012 Mar 8
A cohort study of the prognostic and treatment predictive value of SATB2 expression in colorectal cancer.
Br J Cancer , 2012 Feb 28; 106(5):931-8. Epub 2012 Feb 14
Epitope mapping of antibodies using bacterial surface display.
Nat Methods , 2008 Dec; 5(12):1039-45. Epub 2008 Nov 23
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