Enhanced Validation

Monoclonal Anti-SATB2 Antibody

SATB homeobox 2
References (4)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human SATB2
Alternative Gene Names
FLJ21474, KIAA1034
Open Datasheet
Price
$505.00
Product Number
AMAb90635
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[5 pictures]
Immunohistochemistry analysis in human rectum and skeletal muscle tissues using AMAb90635 antibody. Corresponding SATB2 RNA-seq data are presented for the same tissues.
Show all images
[1 pictures]
Western blot analysis in human cell line HEL.

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
SATB homeobox 2
Target Gene
SATB2
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000038331 (99%)

Rat ENSRNOG00000010188 (99%)

Clonality
Monoclonal
Clone ID
CL0276
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90635 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
SATB homeobox 2
Gene Name
SATB2
Alternative Gene Names
FLJ21474, KIAA1034
UniProt ID
Q9UPW6
Gene (Ensembl)
ENSG00000119042
Entrez Gene ID
Entrez gene: 23314
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Dubois L, Andersson K, Asplund A, Björkelund H

Evaluating real-time immunohistochemistry on multiple tissue samples, multiple targets and multiple antibody labeling methods

BMC Res Notes , 2013 Dec 18; 6:542. Epub 2013 Dec 18

PubMed ID: 24350799 DOI: 10.1186/1756-0500-6-542

Kiflemariam S, Andersson S, Asplund A, Pontén F, Sjöblom T

Scalable in situ hybridization on tissue arrays for validation of novel cancer and tissue-specific biomarkers.

PLoS One , 2012; 7(3):e32927. Epub 2012 Mar 8

PubMed ID: 22412953 DOI: 10.1371/journal.pone.0032927

Eberhard J, Gaber A, Wangefjord S, Nodin B, Uhlén M, Ericson Lindquist K, Jirström K

A cohort study of the prognostic and treatment predictive value of SATB2 expression in colorectal cancer.

Br J Cancer , 2012 Feb 28; 106(5):931-8. Epub 2012 Feb 14

PubMed ID: 22333599 DOI: 10.1038/bjc.2012.34

Rockberg J, Löfblom J, Hjelm B, Uhlén M, Ståhl S

Epitope mapping of antibodies using bacterial surface display.

Nat Methods , 2008 Dec; 5(12):1039-45. Epub 2008 Nov 23

PubMed ID: 19029907 DOI: 10.1038/nmeth.1272

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