Monoclonal Anti-PHGDH Antibody
Lane 2: Human cell line RT-4
Human Protein Atlas
Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000053398 (99%)
Rat ENSRNOG00000019328 (99%)
Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Working concentration: 1 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 2-10 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK
Nature , 2017 Jul 19; 548(7665):112-116. Epub 2017 Jul 19
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