Enhanced Validation

Monoclonal Anti-PDIA3 Antibody

protein disulfide isomerase family A, member 3
References (4)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human PDIA3
Alternative Gene Names
ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC
Open Datasheet
Price
$505.00
Product Number
AMAb90988
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human thyroid gland and skeletal muscle tissues using AMAb90988 antibody. Corresponding PDIA3 RNA-seq data are presented for the same tissues.
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[2 pictures]
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PDIA3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
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[5 pictures]
Immunofluorescence staining in HeLa cell line with Anti-PDIA3 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
protein disulfide isomerase family A, member 3
Target Gene
PDIA3
Belongs to Antibody Panel
Organelle Markers
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000027248 (91%)

Rat ENSRNOG00000015018 (91%)

Clonality
Monoclonal
Clone ID
CL2444
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:20000 - 1:50000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90988 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90988 on the Human Protein Atlas
Protein Name
protein disulfide isomerase family A, member 3
Gene Name
PDIA3
Alternative Gene Names
ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC
UniProt ID
G5EA52
Gene (Ensembl)
ENSG00000167004
Entrez Gene ID
Entrez gene: 2923
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Banfi C, Baetta R, Barbieri SS, Brioschi M, Guarino A, Ghilardi S, Sandrini L, Eligini S, Polvani G, Bergman O, Eriksson P, Tremoli E

Prenylcysteine oxidase 1, an emerging player in atherosclerosis

Commun Biol , 2021 Sep 21; 4:1109. Epub 2021 Sep 21

PubMed ID: 34548610 DOI: 10.1038/s42003-021-02630-z

Kannangara AR, Poole DM, McEwan CM, Youngs JC, Weerasekara VK, Thornock AM, Lazaro MT, Balasooriya ER, Oh LM, Soderblom EJ, Lee JJ, Simmons DL, Andersen JL

BioID reveals an ATG9A interaction with ATG13‐ATG101 in the degradation of p62/SQSTM1‐ubiquitin clusters

EMBO Rep , 2021 Aug 9; 22(10):e51136. Epub 2021 Aug 9

PubMed ID: 34369648 DOI: 10.15252/embr.202051136

Kolpikova EP, Tronco AR, Den Hartigh AB, Jackson KJ, Iwawaki T, Fink SL

IRE1α Promotes Zika Virus Infection via XBP1

Viruses , 2020 Mar 3; 12(3):278. Epub 2020 Mar 3

PubMed ID: 32138181 DOI: 10.3390/v12030278

Mai TT, Hamaï A, Hienzsch A, Cañeque T, Müller S, Wicinski J, Cabaud O, Leroy C, David A, Acevedo V, Ryo A, Ginestier C, Birnbaum D, Charafe-Jauffret E, Codogno P, Mehrpour M, Rodriguez R

Salinomycin kills cancer stem cells by sequestering iron in lysosomes

Nat Chem , 2017 May 16; 9(10):1025-1033. Epub 2017 May 16

PubMed ID: 28937680 DOI: 10.1038/nchem.2778

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