Monoclonal Anti-LMNB1 Antibody

Lamin b1
References (2)
Recommended Applications
Product Description
Monoclonal Antibody against Human LMNB1
Open Datasheet
Price
$505.00
Product Number
AMAb91251
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[6 pictures]
Immunohistochemical staining of human tonsil shows strong nuclear membrane immunoreactivity in lymphoid cells.
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[1 pictures]
Western blot analysis in human cell line MCF-7.
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[5 pictures]
Immunofluorescence staining of MCF7 cells using the Anti-LMNB1 monoclonal antibody, showing specific staining of nuclear membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
Lamin b1
Target Gene
LMNB1
Belongs to Antibody Panel
Organelle Markers
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000024590 (100%)

Rat ENSRNOG00000013774 (100%)

Clonality
Monoclonal
Clone ID
CL3929
Isotype
IgG1
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91251 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91251 on the Human Protein Atlas
Protein Name
Lamin b1
Gene Name
LMNB1
UniProt ID
P20700
Gene (Ensembl)
ENSG00000113368
Entrez Gene ID
Entrez gene: 4001
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Kafkia E, Andres-Pons A, Ganter K, Seiler M, Smith TS, Andrejeva A, Jouhten P, Pereira F, Franco C, Kuroshchenkova A, Leone S, Sawarkar R, Boston R, Thaventhiran J, Zaugg JB, Lilley KS, Lancrin C, Beck M, Patil KR

Operation of a TCA cycle subnetwork in the mammalian nucleus

Sci Adv , 2022 Aug 31; 8(35):eabq5206. Epub 2022 Aug 31

PubMed ID: 36044572 DOI: 10.1126/sciadv.abq5206

Pandit K, Petrescu J, Cuevas M, Stephenson W, Smibert P, Phatnani H, Maniatis S

An open source toolkit for repurposing Illumina sequencing systems as versatile fluidics and imaging platforms

Sci Rep , 2022 Mar 24; 12:5081. Epub 2022 Mar 24

PubMed ID: 35332182 DOI: 10.1038/s41598-022-08740-w

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-LMNB1 (HPA050524)
Anti-LMNB1 (HPA053579)

Questions?

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