Enhanced Validation

Monoclonal Anti-LAMB3 Antibody

laminin, beta 3
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human LAMB3
Alternative Gene Names
BM600-125kDa, kalinin-140kDa, LAMNB1, nicein-125kDa
Open Datasheet
Price
$505.00
Product Number
AMAb91160
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[9 pictures]
Immunohistochemistry analysis in human skin and pancreas tissues using AMAb91160 antibody. Corresponding LAMB3 RNA-seq data are presented for the same tissues.
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[2 pictures]
Western blot analysis of purified human recombinant Laminin-332, Laminin-421, Laminin-511, Laminin-121 and Laminin-221.
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[1 pictures]
Immunofluorescence staining of A-431 cells using the Anti-LAMB3 monoclonal antibody, showing specific staining in the endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
laminin, beta 3
Target Gene
LAMB3
Belongs to Antibody Panel
Laminin Markers
Verified Species Reactivity
Human, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000006025 (76%)

Mouse ENSMUSG00000026639 (75%)

Clonality
Monoclonal
Clone ID
CL3353
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91160 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91160 on the Human Protein Atlas
Protein Name
laminin, beta 3
Gene Name
LAMB3
Alternative Gene Names
BM600-125kDa, kalinin-140kDa, LAMNB1, nicein-125kDa
UniProt ID
Q13751
Gene (Ensembl)
ENSG00000196878
Entrez Gene ID
Entrez gene: 3914
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-LAMB3 (AMAb91161)
Anti-LAMB3 (HPA007584)
Anti-LAMB3 (HPA008069)
Corresponding Antigens
PrEST Antigen LAMB3 (APrEST71223)

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