Enhanced Validation

Monoclonal Anti-LAMA5 Antibody

Laminin subunit alpha 5
References (4)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human LAMA5
Open Datasheet
Price
$505.00
Product Number
AMAb91124
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human placenta and lymph node tissues using AMAb91124 antibody. Corresponding LAMA5 RNA-seq data are presented for the same tissues.
Show all images
[2 pictures]
Western blot analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LAMA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
Laminin subunit alpha 5
Target Gene
LAMA5
Belongs to Antibody Panel
Laminin Markers
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000053691 (68%)

Mouse ENSMUSG00000015647 (62%)

Clonality
Monoclonal
Clone ID
CL3118
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91124 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Protein Name
Laminin subunit alpha 5
Gene Name
LAMA5
UniProt ID
O15230
Gene (Ensembl)
ENSG00000130702
Entrez Gene ID
Entrez gene: 3911
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Sivakumar S, Lieber S, Librizzi D, Keber C, Sommerfeld L, Finkernagel F, Roth K, Reinartz S, Bartsch JW, Graumann J, Müller‐Brüsselbach S, Müller R

Basal cell adhesion molecule promotes metastasis‐associated processes in ovarian cancer

Clin Transl Med , 2023 Jan 16; 13(1):e1176. Epub 2023 Jan 16

PubMed ID: 36647260 DOI: 10.1002/ctm2.1176

Sasamoto Y, Lee CA, Wilson BJ, Buerger F, Martin G, Mishra A, Kiritoshi S, Tran J, Gonzalez G, Hildebrandt F, Jo VY, Lian CG, Murphy GF, Ksander BR, Frank MH, Frank NY

Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation

Cell Rep , 2022 Aug 9; 40(6):111166

PubMed ID: 35947947 DOI: 10.1016/j.celrep.2022.111166

Taniguchi Y, Nagano C, Sekiguchi K, Tashiro A, Sugawara N, Sakaguchi H, Umeda C, Aoto Y, Ishiko S, Rossanti R, Sakakibara N, Horinouchi T, Yamamura T, Kondo A, Nagai S, Nagase H, Iijima K, Miner JH, Nozu K

Clear Evidence of LAMA5 Gene Biallelic Truncating Variants Causing Infantile Nephrotic Syndrome

Kidney360 , 2021 Oct 15; 2(12):1968-1978. Epub 2021 Oct 15

PubMed ID: 35419533 DOI: 10.34067/KID.0004952021

Vaisar T, Hu JH, Airhart N, Fox K, Heinecke J, Nicosia RF, Kohler T, Potter ZE, Simon GM, Dix MM, Cravatt BF, Gharib SA, Dichek DA

Parallel Murine and Human Plaque Proteomics Reveals Pathways of Plaque Rupture

Circ Res , 2020 Jul 30; 127(8):997-1022. Epub 2020 Jul 30

PubMed ID: 32762496 DOI: 10.1161/CIRCRESAHA.120.317295

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Corresponding Antigens
PrEST Antigen LAMA5 (APrEST93815)

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