Enhanced Validation

Monoclonal Anti-HSP90B1 Antibody

heat shock protein 90kDa beta (Grp94), member 1
References (2)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human HSP90B1
Alternative Gene Names
GP96, GRP94, TRA1
Open Datasheet
Price
$505.00
Product Number
AMAb91019
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human prostate and skeletal muscle tissues using AMAb91019 antibody. Corresponding HSP90B1 RNA-seq data are presented for the same tissues.
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[2 pictures]
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSP90B1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
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[5 pictures]
Immunofluorescence staining in HeLa cell line with Anti-HSP90B1 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
heat shock protein 90kDa beta (Grp94), member 1
Target Gene
HSP90B1
Belongs to Antibody Panel
Organelle Markers
Loading Controls
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000020048 (90%)

Rat ENSRNOG00000026963 (87%)

Clonality
Monoclonal
Clone ID
CL2647
Isotype
IgG2b
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:5000 - 1:10000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91019 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91019 on the Human Protein Atlas
Protein Name
heat shock protein 90kDa beta (Grp94), member 1
Gene Name
HSP90B1
Alternative Gene Names
GP96, GRP94, TRA1
UniProt ID
P14625
Gene (Ensembl)
ENSG00000166598
Entrez Gene ID
Entrez gene: 7184
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Miserey‐Lenkei S, Trajkovic K, D'Ambrosio JM, Patel AJ, Čopič A, Mathur P, Schauer K, Goud B, Albanèse V, Gautier R, Subra M, Kovacs D, Barelli H, Antonny B

A comprehensive library of fluorescent constructs of SARS‐CoV‐2 proteins and their initial characterisation in different cell types

Biol Cell , 2021 May 10; 113(7):311-328. Epub 2021 May 10

PubMed ID: 33666950 DOI: 10.1111/boc.202000158

Datta S, Liu Y, Hariri H, Bowerman J, Henne WM

Cerebellar ataxia disease–associated Snx14 promotes lipid droplet growth at ER–droplet contacts

J Cell Biol , 2019 Feb 14; 218(4):1335-1351. Epub 2019 Feb 14

PubMed ID: 30765438 DOI: 10.1083/jcb.201808133

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-HSP90B1 (HPA003901)
Anti-HSP90B1 (HPA008424)
Corresponding Antigens
PrEST Antigen HSP90B1 (APrEST86672)

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