Enhanced Validation

Monoclonal Anti-HDAC5 Antibody

Histone deacetylase 5
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human HDAC5
Alternative Gene Names
FLJ90614, KIAA0600, NY-CO-9
Open Datasheet
Price
$505.00
Product Number
AMAb91501
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[6 pictures]
Immunohistochemistry analysis in human cerebral cortex and liver tissues using AMAb91501 antibody. Corresponding HDAC5 RNA-seq data are presented for the same tissues.
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[1 pictures]
Immunofluorescence staining of RH-30 cells using the Anti-HDAC5 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
Histone deacetylase 5
Target Gene
HDAC5
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000008855 (100%)

Rat ENSRNOG00000020905 (100%)

Clonality
Monoclonal
Clone ID
CL8121
Isotype
IgG1
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:20 - 1:50

Retrieval method: HIER pH6

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91501 on the Human Protein Atlas
Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91501 on the Human Protein Atlas
Protein Name
Histone deacetylase 5
Gene Name
HDAC5
Alternative Gene Names
FLJ90614, KIAA0600, NY-CO-9
UniProt ID
Q9UQL6
Gene (Ensembl)
ENSG00000108840
Entrez Gene ID
Entrez gene: 10014
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-HDAC5 (HPA030991)
Anti-HDAC5 (HPA076420)

Questions?

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