Enhanced Validation

Monoclonal Anti-DICER1 Antibody

dicer 1, ribonuclease type III
References (1)
Recommended Applications
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human DICER1
Alternative Gene Names
Dicer, HERNA, K12H4.8-LIKE, KIAA0928
Open Datasheet
Price
$505.00
Product Number
AMAb90737
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[7 pictures]
Immunohistochemical staining of human uterus shows cytoplasmic immunoreactivity in the glandular epithelium cells.
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[2 pictures]
Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-DICER1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
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[1 pictures]
Immunofluorescence staining of A-431 cells using the anti-DICER1 monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
dicer 1, ribonuclease type III
Target Gene
DICER1
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000041415 (98%)

Rat ENSRNOG00000010711 (98%)

Clonality
Monoclonal
Clone ID
CL0378
Isotype
IgG2a
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90737 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90737 on the Human Protein Atlas
Protein Name
dicer 1, ribonuclease type III
Gene Name
DICER1
Alternative Gene Names
Dicer, HERNA, K12H4.8-LIKE, KIAA0928
UniProt ID
Q9UPY3
Gene (Ensembl)
ENSG00000100697
Entrez Gene ID
Entrez gene: 23405
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Liao CC, Ho MY, Liang SM, Liang CM

Autophagic degradation of SQSTM1 inhibits ovarian cancer motility by decreasing DICER1 and AGO2 to induce MIRLET7A-3P

Autophagy , 2018 Aug 17; 14(12):2065-2082. Epub 2018 Aug 17

PubMed ID: 30081720 DOI: 10.1080/15548627.2018.1501135

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-DICER1 (HPA000694)
Corresponding Antigens
PrEST Antigen DICER1 (APrEST70370)

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