Monoclonal Anti-BRD4 Antibody

bromodomain containing 4
Recommended Applications
Product Description
Monoclonal Antibody against Human BRD4
Alternative Gene Names
CAP, HUNK1, HUNKI, MCAP
Open Datasheet
Price
$505.00
Product Number
AMAb90843
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[4 pictures]
Immunohistochemical staining of human stomach cancer shows moderate to strong nuclear positivity in tumor cells.
Show all images
[1 pictures]
Lane 1: Marker [kDa]
Lane 2: Human cell line U-251 MG
Show all images
[1 pictures]
Immunofluorescence staining of MCF-7 cells using the Anti-BRD4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
bromodomain containing 4
Target Gene
BRD4
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000024002 (76%)

Rat ENSRNOG00000006770 (76%)

Clonality
Monoclonal
Clone ID
CL1118
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90843 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90843 on the Human Protein Atlas
Protein Name
bromodomain containing 4
Gene Name
BRD4
Alternative Gene Names
CAP, HUNK1, HUNKI, MCAP
UniProt ID
O60885
Gene (Ensembl)
ENSG00000141867
Entrez Gene ID
Entrez gene: 23476
Shipping
Normally shipped at ambient temperature.
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-BRD4 (AMAb90841)
Anti-BRD4 (HPA015055)
Anti-BRD4 (HPA061646)

Questions?

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