Enhanced Validation

Monoclonal Anti-BRD4 Antibody

bromodomain containing 4
References (3)
Recommended Applications
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human BRD4
Alternative Gene Names
CAP, HUNK1, HUNKI, MCAP
Open Datasheet
Price
$505.00
Product Number
AMAb90841
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[4 pictures]
Immunohistochemical staining of human stomach cancer shows moderate nuclear positivity in tumor cells.
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[2 pictures]
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-BRD4 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
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[1 pictures]
Immunofluorescence staining of MCF-7 cells using the Anti-BRD4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
bromodomain containing 4
Target Gene
BRD4
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000024002 (76%)

Rat ENSRNOG00000006770 (76%)

Clonality
Monoclonal
Clone ID
CL1115
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90841 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90841 on the Human Protein Atlas
Protein Name
bromodomain containing 4
Gene Name
BRD4
Alternative Gene Names
CAP, HUNK1, HUNKI, MCAP
UniProt ID
O60885
Gene (Ensembl)
ENSG00000141867
Entrez Gene ID
Entrez gene: 23476
Shipping
Normally shipped at ambient temperature.
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Singh AK, Chen Q, Nguyen C, Meerzaman D, Singer DS

Cohesin regulates alternative splicing

Sci Adv , 2023 Mar 1; 9(9):eade3876. Epub 2023 Mar 1

PubMed ID: 36857449 DOI: 10.1126/sciadv.ade3876

Kitagawa T, Kobayashi D, Baron B, Okita H, Miyamoto T, Takai R, Paudel D, Ohta T, Asaoka Y, Tokunaga M, Nakagawa K, Furutani-Seiki M, Araki N, Kuramitsu Y, Kobayashi M

AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing’s sarcoma cells

PLoS One , 2022 Oct 4; 17(10):e0269077. Epub 2022 Oct 4

PubMed ID: 36194562 DOI: 10.1371/journal.pone.0269077

Luo X, Archibeque I, Dellamaggiore K, Smither K, Homann O, Lipford JR, Mohl D

Profiling of diverse tumor types establishes the broad utility of VHL-based ProTaCs and triages candidate ubiquitin ligases

iScience , 2022 Feb 26; 25(3):103985. Epub 2022 Feb 26

PubMed ID: 35295813 DOI: 10.1016/j.isci.2022.103985

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-BRD4 (AMAb90843)
Anti-BRD4 (HPA015055)
Anti-BRD4 (HPA061646)

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