Enhanced Validation

Monoclonal Anti-ANXA1 Antibody

annexin A1
References (2)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human ANXA1
Alternative Gene Names
ANX1, LPC1
Open Datasheet
Price
$505.00
Product Number
AMAb90558
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[6 pictures]
Immunohistochemistry analysis in human placenta and testis tissues using AMAb90558 antibody. Corresponding ANXA1 RNA-seq data are presented for the same tissues.
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[1 pictures]
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ANXA1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
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[1 pictures]
Immunofluorescence staining of A549 cells using the Anti-ANXA1 monoclonal antibody, showing specific staining in the nucleus, cytosol and the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
annexin A1
Target Gene
ANXA1
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000017469 (86%)

Mouse ENSMUSG00000024659 (83%)

Clonality
Monoclonal
Clone ID
CL0199
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90558 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90558 on the Human Protein Atlas
Protein Name
annexin A1
Gene Name
ANXA1
Alternative Gene Names
ANX1, LPC1
UniProt ID
P04083
Gene (Ensembl)
ENSG00000135046
Entrez Gene ID
Entrez gene: 301
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Chen P, Min J, Wu H, Zhang H, Wang C, Tan G, Zhang F

Annexin A1 is a potential biomarker of bone metastasis in small cell lung cancer

Oncol Lett , 2020 Dec 20; 21(2):141. Epub 2020 Dec 20

PubMed ID: 33552260 DOI: 10.3892/ol.2020.12402

Allen KL, Cann J, Zhao W, Peterson N, Lazzaro M, Zhong H, Wu H, Dall’Acqua WF, Borrok MJ, Damschroder MM, Tsui P, Li Q

Upregulation of annexin A1 protein expression in the intratumoral vasculature of human non–small-cell lung carcinoma and rodent tumor models

PLoS One , 2020 Jun 4; 15(6):e0234268. Epub 2020 Jun 4

PubMed ID: 32497150 DOI: 10.1371/journal.pone.0234268

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-ANXA1 (HPA011271)
Anti-ANXA1 (HPA011272)
Corresponding Antigens
PrEST Antigen ANXA1 (APrEST71575)

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