Integrins and Integrin Antibodies

Integrins are receptors for the intracellular matrix. Here you can learn about the function and structure of integrins and find antibodies targeting the different integrins. 

Function and structure of integrins

Integrins are a family of transmembrane receptor proteins, connecting the cytoskeleton and extracellular matrix (ECM). They bind to ECM glycoproteins including collagens, fibronectins, laminins, and a number of cellular receptors. In addition, integrins function as signal transducers. The different integrins activate differential intracellular signaling pathways that control biological and cellular functions including cell adhesion, migration, proliferation, cell differentiation, and apoptosis.

ICC staining of WM-115 cells using monoclonal antibody Anti-ITGB8 (AMAb91467)
ICC staining of WM-115 cells using monoclonal antibody Anti-ITGB8 (AMAb91467) antibody, showing specific staining in the plasma membrane in green.

Integrin specificity

There are 18 alpha and 8 beta subunits, forming at least 24 distinct integrin heterodimers. Each heterodimer consists of a large extracellular domain, binding proteins in the extracellular environment, a single-membrane-spanning transmembrane domain, and a generally small intracellular cytoplasmic tail domain which forms links with the cytoskeletal elements. 

The specificity of integrin binding to ECM components including laminins, collagens, and fibronectin depends on the exposed extracellular domains of the alpha and beta integrin subunits. The integrin proteins are formed by two noncovalently bound alpha and beta subunits. 

A selection of antibodies targeting specific integrin subunits is presented below.  

Antibodies targeting integrin alpha subunits

Our integrin antibodies have been evaluated in one or more applications such as IHC, WB and ICC-IF. The table below shows selected antibodies targeting integrin alpha subunits. For more details on each antibody, click the respective link below and visit the product page.

ITGA1/CD49a : Anti-ITGA1 monoclonal antibody (AMAb91461)

  • Selected interaction partners: ITGB1, LAMA1
  • Involved in: cell adhesion, inflammation, fibrosis

ITGA2/CD49b: Anti-ITGA2 monoclonal antibody (AMAb91469)​

  • Selected interaction partners: ITGB1, LAMA1
  • Involved in: cancer growth/invasion

ITGA3/CD49c: Anti-TGA3 monoclonal antibody (AMAb91446)

  • Selected interaction partners: ITGB1, I TGB4, ITGB5
  • Involved in: Laminin-binding, cancer growth/invasion

ITGA4/CD49d: Anti-ITGA4 polyclonal antibody (HPA074961)​

  • Selected interaction partners: VCAM
  • Involved in: cancer

ITGA5/CD49e : Anti-ITGA5  monoclonal antibody (AMAb91449)​

  • Selected interaction partners: ITGB1, ITGB3, ITGB5, FN1
  • Involved in: cancer growth/invasion

ITGA6/CD49f: Anti-ITGA6 monoclonal antibody (AMAb91450)​

  • Selected interaction partners: ITGB1, ITGB4
  • Involved in: laminin-binding. Cancer invasion/metastasis/stem cells.

ITGA7/ITGA7: Anti-ITGA7 polyclonal antibody (HPA008427)​

  • Selected interaction partners: ITGB1
  • Involved in: laminin-binding, cancer

ITGA8/ITGA8: Anti-ITGA8 monoclonal antibody (AMAb91468)​

  • Selected interaction partners: ITGB1
  • Involved in: epithelial-to-mesenchymal transition.

ITGAD/CD11D: Anti-ITGAD polyclonal antibody (HPA026786)​      

  • Selected interaction partners: ITGB2, ICAM3, VCAM1
  • Involved in: extracellular matrix organization, cell-cell adhesion, immune response

ITGAE/CD103: Anti-ITGAE polyclonal antibody (HPA036313)​        

  • Selected interaction partners: ITGB7, CDH1
  • Involved in: cancer

ITGAM/CD11b: Anti-ITGAM monoclonal antibody (AMAb90911)​

  • Selected interaction partners: ITGB2, ICAM1
  • Involved in: immune system, monocytes

ITGAV/CD51​: Anti-ITGAV polyclonal antibody (HPA004856)​         

  • Selected interaction partners: ITGB1, ITGB3, ITGB5, ITGB6, ITGB8
  • Involved in: cancer

ITGA2B/CD41: Anti-ITGA2B polyclonal antibody (HPA031170)​     

  • Selected interaction partners: ITGB3
  • Involved in: different, including metastasis

ITGAX/CD11c: Anti-ITGAX monoclonal antibody (AMAb90915)​    

  • Selected interaction partners: ITGB2
  • Involved in: cancer, leukemia
IHC stainings of human placenta using Anti-ITGA6 (AMAb91450) and Anti-ITGB4 (AMAb91453).
IHC stainings of human placenta using (left) monoclonal antibody Anti-ITGA6 (AMAb91450) and (right) monoclonal antibody Anti-ITGB4 (AMAb91453) showing an identical pattern. The two integrins interact to form a laminin-binding Alpha6 Beta4 complex involved in cell migration and invasion.

Antibodies targeting integrin beta subunits

Our antibodies targeting the integrins are evaluated in one or more applications such as IHC, WB and ICC-IF. The table below shows selected antibodies for integrin beta subunits. For more details on each antibody, click the respective link below and visit the product page.

ITGB1/CD29: Anti-ITGB1/CD29 polyclonal antibody (HPA059297)

  • Selected interaction partners: ITGA1, ITGA2, ITGA3, ITGA5, ITGA6, ITGA7, ITGA8, ITGA9, ITGA10, ITGA11, ITGAV
  • Involved in: laminin-binding, Cancer growth/invasion

ITGB2/CD18: Anti-ITGB2 polyclonal antibody (HPA016894)

  • Selected interaction partners: ITGAL, ITGAD, ITGAM, ITGAX
  • Involved in: immune system

ITGB3/CD61: Anti-ITGB3 monoclonal antibody (AMAb91470)

  • Selected interaction partners: ITGA4, ITGA5, ITGA2B, ITGAV
  • Involved in: cancer

ITGB4/CD104: Anti-ITGB4 monoclonal antibody (AMAb91454)    

  • Selected interaction partners: ITGA3, ITGA6, SMAD2, SMAD3
  • Involved in: laminin-binding, Cancer growth/invasion

ITGB5/ITGB5: Anti-ITGB5 polyclonal antibody (HPA001820)

  • Selected interaction partners: ITGA3, ITGA5, ITGAV
  • Involved in: cancer, cell adhesion, survival

ITGB6/ITGB6: Anti-ITGB6 polyclonal antibody (HPA023626)

  • Selected interaction partners: ITGAV, MAPK1, FN1
  • Involved in: different diseases, cancer

ITGB8/ITGB8: Anti-ITGB8 monoclonal antibody (AMAb91467)           

  • Selected interaction partners: ITGAV, RHEB, FN1
  • Involved in: cancer
IHC staining of human bone marrow, lymph node, skeletal muscle and spleen using Anti-ITGB2 antibody HPA008877 (left) and Anti-ITGB2 antibody HPA016894 (right)
IHC staining of human bone marrow, lymph node, skeletal muscle and spleen using Anti-ITGB2 antibody HPA008877 (left) and Anti-ITGB2 antibody HPA016894 (right), as part of enhanced validation using independent antibodies, two antibodies targeting different parts of the same protein showing the same staining pattern (validation data pending release).
 IHC staining using monoclonal antibody Anti-ITGA3 (AMAb91446) showing strong immunoreactivity in lung and absence of staining in liver
Enhanced validation using an orthogonal method: IHC staining using monoclonal antibody Anti-ITGA3 (AMAb91446) showing strong immunoreactivity in lung and absence of staining in liver. RNA-Seq data for the same tissues below the image confirm the staining pattern (left). Western blot analysis in U-87 MG cells using the same antibody (right).