Anti-ACSL4 Product Datasheet - Atlas Antibodies -

Product name

Anti-ACSL4

Product number

HPA005552

Lot Number

A27976

Description

acyl-CoA synthetase long-chain family member 4

Alternative names

ACS4, FACL4, LACS4, MRX63, MRX68

Tested applications

Immunohistochemistry-Paraffin Embedded Tissues (IHC)

IHC-P Anti-ACSL4

Immunoperoxidase staining of formalin-fixed, paraffin-embedded human placenta tissue showing strong cytoplasmic staining of trophoblastic cells.

Recommended conditions:

Retrieval method: HIER pH6
Dilution: 1:200

NOTE: Please visit the Human Protein Atlas (www.proteinatlas.org) for the images of the immunohistochemical analysis of 576 human tissue samples of normal and cancer origin as well as 59 cells and cell lines in duplicates.

Western Blot (WB)

WB Anti-ACSL4

Lane 1, Marker [kDa]: 220,112,84,47,32,26,16.8
Lane 2: RT-4
Lane 3: U-251MG sp
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Band of predicted size in kDA (+/- 20%)
with additional bands present

Immunoflouroscence (IF)

IF Anti-ACSL4

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & plasma membrane.

NOTE: Please visit the Human Protein Atlas (www.proteinatlas.org) for the images of the immunofluorescence analysis of three different cell lines stained with the Prestige Antibody as well as with probes, displayed as different colors/channels, targeting specific intracellular compartments.

Protein Array (PA)

Anti-ACSL4 shows specific reactivity against target antigen.

Application notes

Optimal concentrations and conditions for each application should be determined by the end user.

Immunogen

Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence: MNYLEVNRRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLN SEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIV

Vial Size

100 µl

Concentration

0.14 mg/ml

Raised in

Rabbit

Isotype

IgG

Clonality

Polyclonal, mono-specific.

Purity

Affinity purified using the PrEST antigen as affinity ligand.

Species reactivity

Human (Not tested in other species).

Appearance

Clear, colorless solution in phosphate buffered saline, pH 7.2, containing 40% glycerol, and 0.02% sodium azide.

Storage

For continuous use, store at 2-8°C for one-two days. For extended storage, store in -20°C freezer. Working dilution samples should be discarded if not used within 12 hours.

Handling

The antibody solution should be gently mixed before use..

Shipping

Shipped at ambient temperature.

Price

€ 275

Availability

Available in stock