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ANTI-ACSS2

Product name ANTI-ACSS2
Product number HPA004141
Lot Number A08732
Description Acetyl-coenzyme A synthetase, cytoplasmic (EC 6.2.1.1)(Acetate--CoA ligase)(Acyl-activating enzyme)(Acetyl-CoA synthetase)(AceCS)(ACS)(Acyl-CoA synthetase short-chain family member 2)
Database links Ensembl: ENSG00000131069
Uniprot/SWISSPROT;Acc:Q9NR19
Alternative names ACAS2, AceCS, ACS, ACSA, dJ1161H23.1
Tested Applications

Immunohistochemistry-Paraffin Embedded Tissues (IHC-P)

IHC-P ANTI-ACSS2
Hpa004141_t
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Immunoperoxidase staining of formalin-fixed, paraffin-embedded human fallopian tube tissue showing cytoplasmic and/or membranous staining of glandular cells.

Recommended conditions:

  • Retrieval method: HIER pH6
  • Dilution: 1:100

Link to all characterization data on the Human Protein Atlas

NOTE: Please visit the Human Protein Atlas (www.proteinatlas.org) for the images of the immunohistochemical analysis of 576 human tissue samples of normal and cancer origin as well as 59 cells and cell lines in duplicates.

Western Blot (WB)

WB ANTI-ACSS2
HPA004141

Lane 1, Marker [kDa]: 230,110,82,49.3,32.2,25.5,17.6
Lane 2, RT-4
Lane 3, U-251MG sp
Lane 4, Human Plasma
Lane 5, Liver
Lane 6, Tonsil

Band of predicted size in kDA (+/- 20%)
with additional bands present

Link to all characterization data on the Human Protein Atlas

Immunoflouroscence (IF)

IF ANTI-ACSS2
Hpa004141_t
Magnify Click the image to magnify

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & nucleus but not nucleoli.

NOTE: Please visit the Human Protein Atlas (www.proteinatlas.org) for the images of the immunofluorescence analysis of three different cell lines stained with the Prestige Antibody as well as with probes, displayed as different colors/channels, targeting specific intracellular compartments.

Link to all characterization data on the Human Protein Atlas

Protein Array (PA)

ANTI-ACSS2 shows specific reactivity against target antigen.

Application notes Protocols
Optimal concentrations and conditions for each application should be determined by the end user.
Immunogen

Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence:

PGETTQITYHQLLVQVCQFSNVLRKQGIQKGDRVAIYMPMIPELVVAMLA
CARIGALHSIVFAGFSSESLCERILDSSCSLLITTDAFYRGEKLVNLKEL
ADEALQKCQEKGFPVRCCIVVKHLGRAELGMGDSTSQSPPIKRSCPDVQ

Vial Size 100 µl
Concentration 0.11 mg/ml
Raised in Rabbit
Isotype IgG
Clonality Polyclonal, mono-specific.
Purity Affinity purified using the PrEST antigen as affinity ligand.
Species reactivity Human (Not tested in other species).
Appearance Clear, colorless solution in phosphate buffered saline, pH 7.2, containing 40% glycerol, and 0.02% sodium azide.
Material Safety Data Sheet
Storage For continuous use, store at 2-8°C for one-two days. For extended storage, store in -20°C freezer. Working dilution samples should be discarded if not used within 12 hours.
Handling The antibody solution should be gently mixed before use.
Shipping Shipped at ambient temperature.
Price € 275  Freight costs
Availability Available in stock