Quality Assurance
The Atlas Antibodies are mono-specific antibodies, designed to have low cross
reactivity to other human proteins and to work across various application platforms. The mono-specific antibodies
are generated by a strategy where Protein Epitope Signature Tags (PrESTs) are used both as antigens for the development
of polyclonal antibodies and as affinity ligands for the subsequent purification of the antibodies. The PrEST fragments
represent unique regions of the target proteins.
The Atlas Antibodies are tested through a series of quality assurance steps. Plasmid inserts are sequenced to ensure that the correct PrEST antigen sequence is cloned. The size of the resulting recombinant protein is analyzed using mass spectrometry to ensure that the correct antigen has been produced and purified. The affinity purified antibodies are tested for sensitivity and specificity on protein arrays spotted with PrEST antigen fragments. The immunohistochemistry images in normal and disease tissues, cell lines and cell types are annotated and curated by certified pathologists or by specially educated research scientists to assure plausible immunohistochemical staining properties. In addition, the antibodies are tested by Western Blot analysis in a standardized setup as well as by immunofluorescence analysis. The antibodies are then assigned a validation score that assesses all validation tests as well as bioinformatic predictions and literature data.
For more information about the quality control performed in the Human Protein Atlas Project, please visit the HPA Portal
The Atlas Antibodies are tested through a series of quality assurance steps. Plasmid inserts are sequenced to ensure that the correct PrEST antigen sequence is cloned. The size of the resulting recombinant protein is analyzed using mass spectrometry to ensure that the correct antigen has been produced and purified. The affinity purified antibodies are tested for sensitivity and specificity on protein arrays spotted with PrEST antigen fragments. The immunohistochemistry images in normal and disease tissues, cell lines and cell types are annotated and curated by certified pathologists or by specially educated research scientists to assure plausible immunohistochemical staining properties. In addition, the antibodies are tested by Western Blot analysis in a standardized setup as well as by immunofluorescence analysis. The antibodies are then assigned a validation score that assesses all validation tests as well as bioinformatic predictions and literature data.
For more information about the quality control performed in the Human Protein Atlas Project, please visit the HPA Portal